Secretion of T cell receptor fragments from recombinant host cells

ABSTRACT

Variable domain murine T-cell receptor genes have been isolated and used to construct cloning and expression vectors. V α , V β , and single chain V α -V β  fragments have been expressed as secreted domains in  Escherichia coli  using the vectors. The domains are secreted into the culture supernatant in milligram quantities. The single domains and the single chain T-cell receptors are folded into β-pleated sheet structures similar to those of immunoglobulin variable domains. The secreted fragments may be useful for immunization to generate anti-clonotypic antibodies, in vaccination or for high resolution structural studies. The genes encoding these domains may also serve as templates for in vitro mutagenesis and improvement of affinities of the TCR fragments for their interaction with cognate peptide-MHC complexes.

This is a continuing application of U.S. application Ser. No. 08/353,940 filed Dec. 9, 1994, now issued as U.S. Pat. No. 6,399,368 which is a continuation-in-part of U.S. patent application Ser. No. 07/873,930, filed Apr. 24, 1992, now abandoned, which is a continuation-in-part of U.S. patent application Ser. No. 07/822,302, filed Jan. 17, 1992, now abandoned.

BACKGROUND OF THE INVENTION

1. Field of the Invention

The invention relates to cloning vectors useful for the expression of T-cell variable domains, to bacterial cells transformed by the vectors and to methods of producing T-cell variable domains in a prokaryotic host cell, either as single domains or as single chain heterodimers.

2. Description of Related Art

The production of single or heterodimeric T-cell receptor variable domains is of interest for purposes of studying T-cell receptor interaction with antigens and possibly developing approaches to therapies for autoimmune diseases and cancer. An important goal of molecular biology is a detailed understanding at the molecular level of the binding of T-cell receptors to cognate peptide-major histocompatibility complexes. This will be a step in the development, for example, of immunotherapy for T-cell mediated autoimmune disease. Despite this interest and the potential applications arising from the study of T-cell receptor domains, no methods are available for the production of only single T-cell receptor domains, nor has expression and secretion in prokaryotic hosts been successful.

The majority of T cells recognize antigenic peptides bound to class I or II proteins of the major histocompatibility complex (MHC) and are thus “MHC restricted”. The recognition of peptide-MHC complexes is mediated by surface-bound T-cell receptors (TCRs). These receptors are comprised of various heterodimeric polypeptides, the majority of which are α and β polypeptides. A minor population (1-10%) of mature T-cells bear T-cell receptors (TCRS) comprising δ γ heterodimers (Borst et al., 1987; Brenner et al., 1986).

Several composite dimeric species incorporating the α and β polypeptides have been produced in various systems. TCR αβ heterodimers have been expressed as phosphatidyl-inositol linked polypeptides (Lin et al., 1990) or TCR-immunoglobulin chimeras (Gregoire et al., 1991) in mammalian transfectomas. The production of V_(α)Cκ homodimers (Mariuzza & Winter, 1989) and V_(β)-Cβ monomers (Gascoigne, 1990) in mammalian cells has also been described. The expression and secretion of immunoglobulin VH domains (Ward et al., 1989), Fv fragments (Skerra and Pluckthum, 1988; Ward et al., 1989) and Fab fragments (Better et al., 1988) has been reported. Molecular modeling analyses indicate that there are structural similarities between immunoglobulin F_(ab) fragments and the extracellular domains of TCRs (Novotny et al., 1986; Chothia et al., 1988). Several expression systems for the production of recombinant TCRs in mammalian cell transfectomas have been documented but successful expression and secretion of these proteins in a prokaryotic host has not been reported.

Despite apparent expression of a single chain anti-fluorescein TCR in E. coli (Novotny et al., 1991), the product could not be isolated from the periplasm even though the leader sequence had been cleaved from the N-terminus of the recombinant protein. The single chain TCR was relatively insoluble, requiring the use of genetic manipulation to replace five of the “exposed” hydrophobic residues with relatively hydrophilic residues.

No methods are presently available for the production of single or heterodimeric T-cell receptor variable domains as secreted proteins. If available, such species would have potential use in the induction of antibodies as protective vaccines, for the therapy of autoimmune disease, and antibodies for targeting idiotypes (T-cell) or T-cell leukemias. Additionally, secretion of T-cell receptor domains from bacterial cell hosts should provide a convenient, economically attractive and rapid route for production of recombinant T-cell receptors.

Advantages of the production of the TCR variable domains in E. coli compared with expression of phosphatidyl-inositol linked heterodimers and TCR-immunoglobulin chimeras in mammalian cells are the following: (1) E. coli (and other prokaryotic hosts) grow much faster; thus, results of genetic manipulation of the fragments can be analyzed more quickly, (2) use of E. coli is much cheaper than mammalian hosts, (3) production of only the TCR variable domains in mammalian hosts has not been reported. For raising anti-idiotypic antibodies (which recognize variable domains only), this is particularly significant.

TCR fragments have been produced in mammalian cells but they are relatively large. Smaller size TCR segments may allow more rapid structural resolution using such techniques as NMR and X-ray crystallography. Since the variable domains are the regions which interact with peptide-MHC complexes, these regions of TCRs are of considerable interest. Additionally, the use of variable domains alone in immunization should result in the production of anti-variable domain antibodies. Such antibodies are expected to be particularly desirable for use in therapy and diagnosis since they block the interaction of the TCR with antigen and, due to the variable nature of the V_(α)/V_(β) domains or other domains such as V₆₂ and V₆₅ are specific for subsets of T-cells. Large TCR fragments, such as those that can be expressed from mammalian cells, result in production of antibodies not only against the variable domains, but also against the TCR constant domains, (if present in the construct) and/or the immunoglobulin domains (if present in the construct). There would therefore be distinct advantages in having smaller variable domain TCR fragments available, particularly for immunization since any immune response generated is likely to be directed to particular regions of interest, i.e., the V domains.

SUMMARY OF THE INVENTION

The present invention seeks to address one or more of the foregoing problems associated with expression and secretion of T-cell receptor variable domains in a prokaryotic host cell. Recombinant V_(α), V_(β) and single chain V_(α)V_(β) heterodimers have been produced in gram-negative hosts transformed with vectors containing DNA encoding one or more T-cell receptor variable domains. The T-cell receptor domains are efficiently secreted in E. coli or S. marcescens. Only the TCR proteins expressed in E. coli have been characterized by CD. These products contain a high proportion of β-sheet structure indicative of a native structure. Murine T-cell V_(α) and V_(β) domains have been expressed and isolated in yields up to milligram quantities per liter of bacterial culture. Single T-cell variable domains (V_(α) and V_(β)) and single chain (sc) V_(α)V_(β) heterodimers have been produced employing the disclosed vectors.

The recombinant plasmids or expression vectors of the invention are particularly adapted for expression of T-cell receptor domains in transformed prokaryotic host cells. The recombinant plasmids comprise a DNA segment coding for one or more T-cell receptor variable domains. Any of a number of variable domains may be included but preferred domains are the V_(α) and V_(β). Murine T-cell receptor domain V_(α)V_(β) heterodimer, derived from the 1934.4 hybridoma (Wraith et al., 1989) is particularly preferred. Segments of the V_(α) or V_(β) domains as well as other variable domains such as V_(δ)V_(γ), constant domains, C_(α), C_(β1), C_(β2), C_(δ), C_(γ) immunoglobulin CH1, CH2 and CH3 domains, etc. may also be employed. It is also contemplated that variations of T-cell receptor variable domains also fall within the scope of the invention. Such variations may arise from mutations such as point mutations and other alterations affecting one or more amino acids or the addition of amino acids at the N or C termini. While the invention has been illustrated with murine T-cell receptors, similar strategies are applicable to the receptor domains from other species, including rat, man and other mammals.

Other DNA segments may also be included linked to the variable domains described, for example, one or more recombinant T-cell receptor variable domains of one or more specificities linked to TCR constant domains, immunoglobulin constant domains, or bacteriophage coat protein genes. Once expressed, any of the products herein could be radiolabelled or fluorescently labeled, or attached to solid supports, including sepharose or magnetic beads or synthetic bilayers such as liposomes. The products could also be linked to carrier proteins such as bovine serum albumin. The TCR V domains, or V domains linked to other proteins (such as constant domains), could also be linked synthetically to co-receptors such as the extracellular domains of CD4 or CD8. This could increasae the avidity of the interaction of the TCR fragment with cognate peptide MHC complexes.

Cloning vectors are included in one aspect of the present invention. The vectors include a leader sequence, preferably pelB (Better et al., 1988), although other leader sequences may be used, for example, alkaline phosphatase (phoA) or ompA. In a preferred embodiment, the pelB leader segment is modified with a unique restriction site, such as NcoI, allowing insertion of TCR variable domain genes. Introduction of such restriction sites is a convenient means of cloning in a DNA segment in the same reading frame as the leader sequence.

Modification of the leader sequence DNA may be achieved by altering one or more nucleotides employing site-directed mutagenesis. In general, the technique of site specific mutagenesis is well known in the art as exemplified by publications (Carter, et al., 1985). As will be appreciated, the technique typically employs a phage vector which exists in both a single stranded and double stranded form. Typical vectors useful in site directed mutagenesis include vectors such as the M13 phage (Messing, et al., 1981). These phage are readily commercially available and their use is generally well known to those skilled in the art.

Site directed mutagenesis in accordance herewith is performed by first obtaining a single stranded vector which includes within its sequence the DNA sequence encoding a leader sequence, pelB being used herewith. An oligonucleotide primer bearing the desired mutated sequence is prepared, generally synthetically, for example by the method of Cray, et al. (1978). The primer is annealed with the single stranded vector and subjected to DNA polymerizing enzymes such as the E. coli polymerase I Klenow fragment. In order to complete the synthesis of the mutation bearing strand, a heteroduplex is formed wherein one strand encodes the original non-mutated sequence and the second strand bears the desired mutation. The heteroduplex may be transformed into a bacterial such as E. coli. or S. marcescens cells used herein. Clones are screened using colony hybridization and radiolabelled mutagenic oligonucleotide to identify colonies which contain the mutated plasmid DNA (Carter et al., 1985).

Constructs may also include a “tag” useful for isolation and purification of the expressed and secreted polypeptide product. Tags are relatively short DNA segments fused in-frame with a sequence encoding a desired polypeptide, such as the TCR variable domains herein described, which have the function of facilitating detection, isolation and purification. For example, affinity peptides may be encoded by the segments, allowing isolation by selective binding to specific antibodies or affinity resins. Any of a number of tags may be used, including the c-myc tag, (his)₆ tag, decapeptide tag (Huse et al., 1989), Flag™ (Immunex) tags and so forth. A number of the tags are also useful for the detection of expressed protein using Western blotting (Ward et al., 1989; Towbin et al., 1978).

(His)₆ tags, for example, are preferable for purifying secreted polypeptide products on affinity metal chromatography columns based on metals such as Ni²⁺. The (his)₆ peptide chelates Ni²⁺ ions with high affinity. Polypeptide products containing these residues at the N or C termini bind to the affinity columns, allowing polypeptide impurities and other contaminants to be washed away as part of the purification process. Polypeptide products can then be eluted from the column with high efficiency using, for example, 250 mM imidazole.

Peptide tags, or linkers, may also be incorporated into the TCR product. For single chain TCR fragments, preferred linker peptides include a 15-mer, for example, (gly₄ser)₃ or other linkers, such as those described in Filpula and Whitlow (1991).

The invention has been illustrated with prokaryotic host cells, but this is not meant to be a limitation. The prokaryotic specific promoter and leader sequences described herein may be easily replaced with eukaryotic counterparts. It is recognized that transformation of host cells with DNA segments encoding any of a number of T-cell variable domains will provide a convenient means of providing fully functional TCR protein. Both cDNA and genomic sequences are suitable for eukaryotic expression, as the host cell will, of course, process the genomic transcripts to yield functional mRNA for translation into protein.

It is similarly believed that almost any eukaryotic expression system may be utilized for the expression of TCR proteins, e.g., baculovirus-based, glutamine synthase based or dihydrofolate reductase-based systems could be employed. Plasmid vectors would incorporate an origin of replication and an efficient eukaryotic promoter, as exemplified by the eukaryotic vectors of the pCMV series, such as pCMV5.

For expression in this manner, one would position the coding sequences adjacent to and under the control of the promoter. It is understood in the art that to bring a coding sequence under the control of such a promoter, one positions the 5′ end of the transcription initiation site of the transcriptional reading frame of the protein between about 1 and about 50 nucleotides “downstream” of (i.e., 3′ of) the chosen promoter.

Where eukaryotic expression is contemplated, one will also typically desire to incorporate into the transcriptional unit, an appropriate polyadenylation site (e.g., 5′-AATAAA-3′) if one was not contained within the original cloned segment. Typically, the poly A addition site is placed about 30 to 2000 nucleotides “downstream” of the termination site of the protein at a position prior to transcription termination.

As used herein the term “engineered” or “recombinant” cell is intended to refer to a cell into which a recombinant gene, such as a gene encoding a T-cell receptor variable domain, has been introduced. Therefore, engineered cells are distinguishable from naturally occurring cells which do not contain a recombinant gene that is introduced by transfection or transformation techniques. Engineered cells are thus cells having a gene or genes introduced through the hand of man. Recombinantly introduced genes will either be in the form of a cDNA (i.e., they will not contain introns), a copy of a cDNA gene, genomic DNA (with or without introns; for expression in prokaryotic hosts, the DNA should be without introns), or will include DNA sequences positioned adjacent to a promoter not naturally associated with the particular introduced gene.

Generally speaking, it may be more convenient to employ as the recombinant gene a cDNA version of the gene. It is believed that the use of a cDNA gene will provide advantages in that the size of the gene is generally much smaller and more readily employed to transform (or transfect) a targeted cell than a genomic gene, which will typically be up to an order of magnitude larger than the cDNA gene. However, the inventor does not exclude the possibility of employing a genomic version of a particular gene where desired, for expression in mammalian cells. For prokaryotic host cells, constructs without introns will be used, since prokaryotes do not splice introns and exons into functional mRNA.

Suitable host cells useful in the practice of the invention include gram-negative organisms and might include Serratia marcescens, Salmonella tymphinurium and similar species. A particularly preferred host cell is E. coli and the several variants of E. coli that are readily available and well known to those of skill in the art.

A particular aspect of the invention is a method for the production of T-cell receptor variable domains. A gram-negative microorganism host cell is transformed with any of the disclosed recombinant vectors cultured in an appropriate bacterial culture medium to produce T-cell receptor variable domains which are subsequently isolated. Culturing typically comprises both a growing and an induction step. Growing is conveniently performed in such media as Luria broth plus 1% glucose, 4×TY (double strength 2×TY) plus 1% glucose, minimal media plus casamino acids and 5% w/v glycerol with temperatures in the range of 20° C. to about 37° C., preferably between 25-30° C. All media contains ampicillin at a concentration of 0.1-1 mg/ml; to select bacterial cells which contain the expression plasmid. Induction of expression is typically performed at a point after growth has been initiated, usually after 12-16 hours at 30° C. This length of growth results in the cells being in the early stationary phase at the induction stage. If the growth media contains glucose, the cells are pelleted and washed prior to addition of inducer (isopropylthiogalactopyranoside (IPTG) at a concentration of 0.1-1 mM) since glucose inhibits induction or expression. Cells may be grown for shorter periods prior to induction, for example for 6-10 hours, or to the mid-exponential stage of growth. Cells are induced for 5-28 hours. 5-6 hours of induction is a preferred induction time if the protein is to be isolated from the periplasm, since longer induction times result in the protein leaking into the culture supernatant. However, it may be desirable to isolate product from the external medium, in which case one would prefer using longer induction times. Temperatures in the range of 20° C. to 37° C. may be used as growth and induction temperatures, with 25° C. being a preferred induction temperature.

Isolating polypeptide products produced by the microbial host cell and located in the periplasmic space typically involves disrupting the microorganism, generally by such means as osmotic shock, sonication or lysis. Once cells are disrupted, cells or cell debris may be conveniently removed by centrifugation or filtration, for example. The proteins may be further purified, for example, by affinity metallic resin chromatography when appropriate peptide tags are attached to the polypeptide products.

Alternatively, if the induction period is longer than 8 hours (at 25° C., for example), so that the protein leaks into the culture supernatant, cells may be removed from the culture by centrifugation and the culture supernatant filtered and concentrated (for example, 10-20 fold). Concentrated supernatant is then dialyzed against phosphate buffered saline and separation achieved by column chromatography, such as affinity or adsorption chromatography. An example is separation through Ni²⁺-NTA-agarose to separate appropriately tagged proteins such as those carrying a (his)₆ tag. When these tags are used in the construction of an expression vector, histidine tags are particularly preferred as they facilitate isolation and purification on metallic resins such as Ni⁺²-NTA agarose.

Also contemplated within the scope of the invention are the recombinant T-cell receptor single-chain variable domain products. These include single chain heterodimers comprising the variable domains V_(α), V_(β), V_(γ) and V_(δ). However, it will be appreciated that modification and changes may be made in the composition of these domains, for example by altering the underlying DNA, and still obtain a molecule having like or otherwise desirable characteristics.

In general, certain amino acids may be substituted for other amino acids in a protein structure without appreciable loss of interactive binding capacity with structures such as, for example, antigen-binding regions of antibodies or receptor sites. Since it is the interactive capacity and nature of a protein that defines that protein's biological functional activity, certain amino acid sequence substitutions can be made in a protein sequence (or, of course, its underlying DNA coding sequence) and nevertheless obtain a protein with like or even countervailing properties (e.g., antagonistic v. agonistic). It is thus contemplated by the inventor that various changes may be made in the coding sequence for the T-cell variable domains without appreciable loss of the biological utility or activity of the encoded protein. It may even be possible to change particular T-cell receptor variable domain residues and increase the interactive ability, i.e., binding affinity of the variable domains for cognate peptide MHC complex.

In making such changes, the hydropathic index of amino acids may be considered. The importance of the hydropathic amino acid index in conferring interactive biologic function on a protein is generally understood in the art (Kyte et al., 1982). It is known that certain amino acids may be substituted for other amino acids having a similar hydropathic index or score and still retain a similar biological activity. Each amino acid has been assigned a hydropathic index on the basis of its hydrophobicity and charge characteristics, these are: isoleucine (+4.5); valine (+4.2); leucine (+3.8); phenylalanine (+2.8); cysteine/cystine (+2.5); methionine (+1.9); alanine (+1.8); glycine (−0.4); threonine (−0.7); serine (−0.8); tryptophan (−0.9); tyrosine (−1.3); proline (−1.6); histidine (−3.2); glutamate (−3.5); glutamine (−3.5); aspartate (−3.5); asparagine (−3.5); lysine (−3.9); and arginine (−4.5).

It is believed that the relative hydropathic character of the amino acid may play a role in determining the secondary structure of the resultant protein, which in turn defines the interaction of the protein with other molecules, for example, enzymes, substrates, receptors, antibodies, antigens, and the like. It is known in the art that an amino acid may be substituted by another amino acid having a similar hydropathic index and still obtain a biological functionally equivalent protein. In such changes, the substitution of amino acids whose hydropathic indices are within ±2 is preferred, those which are within ±1 are particularly preferred, and those within ±0.5 are even more particularly preferred. It is also conceivable that it may be possible to increase the binding affinity of a T-cell receptor variable domain by changing an amino acid to another which is quite different in hydrophobicity. This may not have an adverse effect on the structure of the protein, since the residues which interact with peptide-MHC complexes are believed to be located in the exposed hypervariable loops of the V domains.

Substitution of like amino acids can also be made on the basis of hydrophilicity, particularly where the biological functional equivalent protein or peptide thereby created is intended for use in immunological embodiments. U.S. Pat. No. 4,554,101, incorporated herein by reference, states that the greatest local average hydrophilicity of a protein, as governed by the hydrophilicity of its adjacent amino acids, correlates with its immunogenicity and antigenicity, i.e., with a biological property of the protein.

As detailed in U.S. Pat. No. 4,554,101, the following hydrophilicity values have been assigned to amino acid residues: arginine (+3.0); lysine (+3.0); aspartate (+3.0±1); glutamate (+3.0±1); serine (+0.3); asparagine (+0.2); glutamine (+0.2); glycine (0); (0±1); threonine (−0.4); alanine (−0.5); histidine (−0.5); cysteine (−1.0); methionine (−1.3); valine (−1.5); leucine (−1.8); isoleucine (−1.8); tyrosine (−2.3); phenylalanine (−2.5); tryptophan (−3.4). It is understood that an amino acid can be substituted for another having a similar hydrophilicity value and still, although not always, obtain a biologically equivalent, and in particular, an immunologically equivalent protein. In such changes, the substitution of amino acids whose hydrophilicity values are within ±2 is preferred, those which are within ±1 are particularly preferred, and those within ±0.5 are even more particularly preferred.

Amino acid substitutions are generally therefore based on the relative similarity of the amino acid side-chain substituents, for example, their hydrophobicity, hydrophilicity, charge, size, and the like. Exemplary substitutions which take various of the foregoing characteristics into consideration are well known to those of skill in the art and include: arginine and lysine; glutamate and aspartate; serine and threonine; glutamine and asparagine; and valine, leucine and isoleucine.

While discussion has focused on functionally equivalent polypeptides arising from amino acid changes, it will be appreciated that these changes may be effected by alteration of the encoding DNA; taking into consideration also that the genetic code is degenerate and that two or more codons may code for the same amino acid.

As illustrated herein, transformed E. coli host cells will provide good yields of T-cell receptor variable domains. The yields of about 1-2 mg/L for V_(α), 0.1-0.2 mg/L for V_(β) and 0.5-1 mg/L for the single chain V_(α)V_(β) heterodimer may be readily scaled up to produce relatively large quantities of these TCR domains in a matter of days, employing, for example, a (his)₆ tag for affinity purification with the Ni²⁺-NTA-agarose. Thus the expression system will provide a valuable source of soluble TCR protein for use in immunizations for the generation of anti-clonotypic antibodies, useful, for example, in passive immunization for the treatment of disease. As an example, TCRs expressed on the surface of leukemic T-cells could be expressed as soluble domains and used in immunization to generate anti-TCR antibodies. Such antibodies could be used as targeting reagents in the therapy of T-cell leukemias. It is also conceivable that such soluble TCRs (derived from pathogenic T cells) may be used in vaccination to generate a specific anti-TCR response in vivo for the therapy of autoimmune diseases in a similar way to that reported using peptides derived from TCR V-regions (Vandenbark, et al., 1989; Howell, et al., 1989; Offner, et al., 1991). Moreover, recombinant V_(α), V_(β), V_(δ), V_(γ), single chain V_(α)V_(β) fragments, domains, or even subfragments thereof, are potentially useful for mapping the TCR residues which are functionally important in binding peptide-MHC complexes.

The present invention shows that scTCR (from V_(α) and V_(β)) derived from the 1934.4 T-cell hybridoma (Wraith et al., 1989) is secreted into the periplasm and may be purified in yields of approximately 0.5-1 mg/L culture using Ni²⁺-NTA-agarose. FIG. 2 shows an SDS polyacrylamide gel analysis of the purification of this protein. For the scTCR in particular, lower growth and induction temperatures of 25-30° C. resulted in higher expression yields. Even higher expression may be achieved with modifications to growth medium and temperature, as recognized by those of skill in the art. For example, lower growth and induction temperatures were found to enhance expression of other recombinant proteins in E. coli (Takagi et al., 1988).

Purification of the herein described murine TCR variable domains may be achieved in many ways, including chromatography, density gradient centrifugation and electrophoretic methods. A particular example of scTCR purification employs an affinity column, made by linking the monoclonal antibody KJ16 (specific for murine V_(β)8: Kappler et al., 1988) to sepharose. For the 1934.4 derived scTCR, purification with this affinity column indicated that the epitope recognized by this monoclonal antibody is in the correct conformational state in the recombinant scTCR.

A rapid method for the production of soluble, heterodimeric TCRs, as presented in the present disclosure, may be readily extended to the production of soluble TCRs of different specificities, derived from other species such as man. This opens up new avenues for immunotherapy and diagnosis, particularly in relation to T cell mediated autoimmune diseases and T-cell leukemias. Also contemplated is the use of random in vitro mutagenesis to alter the residues of recombinant TCR fragments, and to express these fragments as either soluble proteins as disclosed herein or on the surface of bacteriophage (McCafferty et al., 1990). Mutants binding with higher affinity to peptide-MHC complexes may be screened for or selected for using solid surfaces coated with antigen presenting cells and cognate peptide. Such higher affinity mutants would have a large number of applications, for example, in therapy of autoimmune disease as blocking reagents. The TCR fragments are produced in sufficient quantities for a wide variety of tests and studies including, for example, high resolution structural analyses with NMR and X-ray diffraction.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows the construction of plasmids for expression of the V_(α) domain (V_(α)pelBtag1), V_(β) domain (V_(β)pelBtag1) and co-expression of the two domains (V_(α)V_(β)pelBtag1). H=HindIII, N=NcoI, B=BstI and E=EcoRI. Filled in box=pelB leader, stippled box=V_(β) gene, striped box=five 3′ codons of VH gene in pSWI-VH-poly-tag1 plus tag1 (c-myc) codons, open box=V_(α) gene and open circle=lacZ promoter.

FIG. 2 shows the expression analysis of V_(α) and V_(β) domains tagged with carboxy terminal c-myc peptides by western blotting (using the 9E10 monoclonal antibody which recognizes the c-myc epitope) of culture supernatants electrophoresed) on a 15% SDS polyacrylamide gel. E. coli recombinants harboring the following plasmids were analyzed: lane 1, V_(α)V_(β)pelBtag1; lane 2, VβpelBtag1, and lane 3, V_(α)pelBtag1. The mobilities of molecular weight size standards, run on an equivalent gel stained with Coomassie brilliant blue rather than transferred onto nitrocellulose, are indicated in kDa on the right margin.

FIG. 3 shows the strategy used for construction of plasmids for expression and purification of single V_(α) and V_(β) domains and scTCR V_(α) V_(β) fragments.

FIG. 4 shows the strategy for the construction of scV_(α)V_(β)pelBHis ver.2. The pelB leader is represented by a stippled box, the V_(α) and V_(β) domains by open boxes, the single chain (sc) linker peptide by vertical lines and the (his)₆ tag by a hatched box. The lacZ promoter is represented by an open circle. Oligonucleotides A and B are set forth in Example 1. PCR with oligonucleotide B results in the deletion of two codons between the 3′ end of J_(α) and the 5′ end of the (gly₄ser)₃ linker peptide.

FIG. 5 shows the nucleic acid and derived amino acid sequence of V_(α) TCR gene with the (his)₆ tag.

FIG. 6 shows the nucleic acid and derived amino acid sequence of V_(β) TCR gene with the (his)₆ tag.

FIGS. 7A and 7B show the nucleic acid and derived amino acid sequence of scV_(α)V_(β) with the (his)₆ tag.

FIG. 8 shows a schematic representation of the plasmid construct for scTCR.

FIGS. 9A and 9B show the nucleic acid and derived amino acid sequence of scV_(α)V_(β)pelBhis ver. 2.

FIG. 10 shows the SDS PAGE analysis of the purified single domains (lanes 2-7) and scTCR (lanes 8-10). Lane 1: molecular weight markers (with sizes shown on the left margin in kDa; lane 2, osmotic shock fraction of E. coli harboring V_(α)pelBhis; lane 3, flowthrough from V_(α)pelBhis osmotic shock fraction after passage through Ni²⁺-NTA agarose column: lane 4, purified V_(α) domain; lanes 5-7, same as lanes 2-4 respectively, except that E. coli harbors V_(β)pelBhis; lanes 8-10, same as lanes 2-4, respectively except that E. coli harbors scV_(α)V_(β)pelBhis.

FIGS. 11A and 11B show the circular dichroism spectra for the recombinant TCR proteins. Panel A: spectrum for the V_(α) domain is represented by a solid line; V_(β) by a broken line; and scTCR by a dashed and dotted line. Panel B: spectrum for the D1.3 scFv fragment. All spectra were smoothed and baseline corrected.

DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS

The present invention concerns the cloning and expression of the variable domains of a murine T-cell receptor (TCR) in a gram-negative bacterial host. The genes encoding the TCR for V_(α) and V_(β) domains of T-cell hybridoma 1934.4 (Wraith et al., 1989) were isolated using the polymerase chain reaction (Saiki et al., 1988) and ligated into expression vectors constructed from pUC119 (Viera et al., 1987). PUC119 contains a lacZ promoter sequence upstream of the site into which the V_(α) and V_(β) domain genes were ligated. The new expression vectors included a pelB leader segment in translational frame with the cloned variable domain genes. Modification of these expression vectors, containing V_(α) and V_(β). single variable domains, was accomplished by cloning a V_(β). encoding segment 3′ to a V_(α) gene segment and incorporating a single chain linker peptide. Bacterial hosts transformed with this construct were capable of expressing single chain heterodimeric TCR V_(α)V_(β) domains.

The TCR genes employed were derived from a pathogenic CD4⁺ T-cell clone associated with induction of experimental allergic (autoimmune) encephalomyelitis (EAE) in the H-2^(u) mouse (Wraith et al., 1989). EAE is a prototypic model of T-cell mediated autoimmune disease and may be a valuable model for multiple sclerosis in humans.

Expression of Single V_(α) and V_(β) Domains

Vα and V_(β) genes derived from 1934.4 cells were cloned into VHNco-poly-tag1 to generate V_(α)pelBtag1 (V_(α) gene only) and V_(β)pelBtag1 (V_(β) gene only) and transformed into E. coli host cells. The nucleotide sequences of the constructions were confirmed by DNA sequencing prior to growing up and inducing E. coli recombinants for expression. Culture supernatants were analyzed by western blotting which clearly showed that the V₆₀ and Vβ domains were expressed individually and could be secreted into the culture supernatant. The molecular weights, from SDS gel analysis, were estimated as 17 kDa (V_(α)-tag1) and 14.5 kDa (V_(β)-tag1). For the V_(α) domain this is significantly higher than that predicted by amino acid analysis, but is similar to the anomalously low gel mobilities observed for single antibody VH domains. The level of secretion of the V_(α) domain was particularly high and was similar to, if not greater than, that reported for immunoglobulin FvD1.3 fragment expressed and secreted from E. coli (Ward et al., 1989). The level was estimated at 10 mg per liter of culture, by comparison with culture supernatants of E. coli recombinants harboring pSW1-VHD1.3-VkD1.3-tag1 using western blotting. The relatively high expression level of the V_(α) domain may reflect a propensity of this domain to form homodimers. Such homodimer formation could mask the hydrophobic residues of the V_(α) domain which, in a native TCR, interact with analogous Vβ residues during V_(α):V_(β) pairing, thus increasing the solubility (and secretion levels) of the homodimer.

In contrast to the V_(α) domain, the V_(β) domain was secreted into the culture supernatant at levels of about 0.5-1 mg per liter of culture, although the intracellular/periplasmic levels of the V_(β) domain were similar to those of the V_(α) domain. The V_(β) protein apparently does not fold into a soluble form as readily as the V_(α) domain. It is expected that the amount of secreted V_(β) will be increased by altering the induction conditions. Alternatively, higher levels of soluble Vβ domain may be obtained by osmotically shocking the recombinant E. coli cells, followed by denaturation and refolding of the released V_(β) protein.

Co-Expression of V_(α) and V_(β) Domains

As illustrated in Example 2, the V_(α) and V_(β) domains may be co-expressed and secreted from E. coli recombinants harboring V_(α)V_(β)pelBtag1. The V_(α) polypeptide was secreted in excess over the V_(β) domain, indicating that at least some of the recombinant TCR protein is not heterodimeric. However, V_(α) domain secretion levels were lower when co-expressed with the V_(β) polypeptide than when expressed and secreted as a single domain. This may be due, for example, to limitations on the amount of protein which can be secreted into the E. coli periplasm, i.e., V_(β) secretion may compete with V_(α) secretion. Alternatively, there may be some polarity effects on the expression of the V_(α) domain, which is 3′ to the V_(β) gene in V_(α)VβpelBtag1.

The present invention demonstrates that TCR V_(α) and V_(β) polypeptides can be expressed and secreted from recombinant E. coli cells as either individual domains or co-expressed. The secretion system may be employed as a rapid and economically favorable alternative to existing methods for the production of TCRs or TCR-immunoglobulin chimeras in mammalian cell transfectomas.

For purification, the V_(α) and V_(β) domains were expressed with carboxy terminal (his)₆ tags. As a preferred method of purification, induction conditions were established allowing isolation of the protein from the periplasmic space using osmotic shock. The osmotic shock fractions were dialyzed against phosphate buffered saline overnight at 4° C. with 3 changes, and the dialysate passed through an Ni²⁺-NTA-agarose column. Alternatively, longer induction times were used and the protein purified from the culture supernatant. Concentration of the supernatant was then effected by concentration under high pressure using an Amicon filtration unit followed by overnight dialysis against PBS and passage through a Ni²⁺-NTA-agarose column. Using purification from osmotic shock fractions, yields of 1-2 mg/L V_(α) domain and 0.1-0.2 mg/L V_(β) were obtained.

The V_(α) and V_(β) domains do not associate when co-expressed within the same bacterial cell. To drive the association of the two domains, therefore, the V_(α) domain was linked to the V_(β) domain by a synthetic linker and the two domains expressed as a heterodimeric scTCR fragment. This heterodimer may be expressed with carboxy-terminal (his)₆ peptide tags and purified using affinity purification on Ni²⁺-NTA-agarose columns. The purification yields from osmotic shock fractions were 0.5-1 mg/L culture.

To assess the folded state of the recombinant TCR fragments, CD spectral analyses were carried out on the fragments and on the D1.3 single chain Fv fragment. The minima in the curves at 218 nm for these proteins indicate the presence of a high proportion of β-pleated sheet structure (Johnson, 1990). These spectra also indicated a lack of α-helical regions, since a-helical regions result in minima at ˜208 nm and 224 nm, and this is consistent with the proposed structural models for TCR extracellular domains (Novotny et al., 1986; Chothia et al., 1988), and the structure of the crystallographically solved D1.3 Fv fragment (Bhat et al., 1990). The maxima at approximately 205 nm in the spectra of the V_(α) domain and the D1.3Fv fragment has previously been associated with the presence of an abundance of β-turns.

Overall and in general terms the invention shows that single V_(α), V_(β) domains and single chain heterodimeric TCRs (scTCRs) derived from an encephalitogenic T cell hybridoma may be expressed and purified in yields ranging from 0.1-2 milligrams per liter of bacterial culture. In addition, structural analysis using CD indicates that the recombinant TCR fragments contain a high proportion of β pleated sheet structures. Although molecular modelling has indicated that the extracellular domains of TCRs may resemble immunoglobulin Fv and Fab fragments in structure (Novotny et al., 1986; Chothia et al., 1988), to date this has not been demonstrated empirically. The ability of the V_(α), V_(β) domains and heterodimeric scTCR to inhibit the binding of the 1934.4 T cell hybridoma to cognate peptide-MHC complexes (N-terminal residues 1-11 of myelin basic protein associated with the MHC class II protein I-A^(u); Wraith et al., 1989) is of particular interest because it would demonstrate functional activity of the recombinant proteins. It is conceivable, however, that soluble TCR fragments are ineffective inhibitors of the multivalent, high avidity, interaction (Harding and Unanue, 1990) of T cell borne antigen receptors with peptide-MHC complexes. The tripartite interaction of ‘native’ TCR on CD4+ T cells with peptide-MHC complexes may be stabilized by contacts between CD4 residues and the MHC class II molecule (Sleckman et al., 1987: Fleury et al., 1991). The absence of this ‘co-receptor’ in the recombinant TCRs may therefore decrease the avidity of the interaction further.

Materials and Methods

Bacterial Strains and Plasmids E. coli BMH71-18 was used as host for the cloning and expression of TCR domains (Rüther et al., 1981). Plasmid pSWI-VH-poly-tag1 (Ward et al., 1989) was modified by replacing pUC19 with pUC119 (Viera et al., 1987) as the backbone vector. In addition, an NcoI restriction site was inserted into the pelB leader sequence using site directed mutagenesis to generate V_(H)Nco-poly-tag1.

Isolation of V_(α) and V_(β) Genes

The V_(α) and V_(β) genes were isolated from 1934.4 hybridoma cells (Dr. D. Wraith, Cambridge University, Department of Pathology, Immunology Division, Level 3 Laboratories Block, Addenbrookes's Hospital, Hills Road, Cambridge CB2 2QQ, UK) using a PCR amplification method. 10⁶ cells were washed once in sterile PBS, then resuspended in 1 ml sterile deionized water and heated at 100° C. for 5 mins. This results in isolation of genomic DNA. Debris was pelleted by centrifugation for 3 minutes at room temperature at 11,000 rpm and 2-10 μl of supernatant used in a PCR reaction with the following V_(α) or V_(β) specific primers:

V_(α): I: 5′-ATC CTT CCA TGG CCG ACT CAG TGA CTC AGA CGG AAG GT-3′ II: 5′-AAG GAT GGT GAC CGG TTT ATT GGT GAG TTT GGT TCC-3′ V_(β): III: 5′-ATC CTT CCA TGG CCG AGG CTG CAG TCA CCC AAA GTC CA-3′ IV: 5′-AAG GAT GGT GAC CAG AAC AGT CAG TCT GGT TCC TGA-3′

For each domain, the oligonucleotides encode either an NcoI or BstEII fragment (underlined) to allow restriction enzyme digestion of the PCR products, followed by gel purification using “Geneclean” (BIO 101, Valley Park, Mo. 63088) and ligation as an NcoI-BstEII fragment into VH_(H)Nco-poly-tag1.

PCR conditions were as follows:

3 units Promega Taq polymerase (Promega, Madison, Wis. 53711-5399)

5 μl 10× Promega reaction buffer

25 pmol of each oligonucleotide primer

0.2 mM dNTPs

2-10 μl 1934.4 hybridoma supernatant (crude genomic DNA preparation)

Water to 5 μl

Cycling conditions were 94° C. for 0.5 min, 55° C. for 0.5 min, 72° C. for 1 min with Taq polymerase added at the end of the first cycle, that is, at 72° C. Thirty cycles of PCR were conducted and an additional 3 units of Taq polymerase added after 15 cycles to minimize occurrence of PCR errors. Alternatively, less error-prone polymerases such as Vent™ polymerase (New England Biolabs, Beverly, Mass. 01915-5599) may be used.

The following examples are intended to illustrate the practice of the present invention and are not intended to be limiting. Although the-invention is demonstrated with variable murine T-cell V_(α) and V_(β) domains, other domains will be adaptable to similar constructs as those described hereinabove. Likewise, a variety of tags, linker sequences and leader sequences may be employed depending on the particular purification or isolation methods desired to obtain the polypeptide products.

EXAMPLE 1

The following example illustrates the construction of plasmids for expression of the T-cell receptor single domains, V_(α) and V_(β) and the single chain V_(α)V_(β) construct. Two types of plasmids are illustrated; one with a c-myc tag and the other with a (his)₆ tag. Other tags could be used.

V_(α)pelBtag1 Plasmid

The tag portion for the plasmid construct used in this example is c-myc with the following nucleic acid sequence: GAA CAA AAA CTC ATC TCA GA AGA GGA TCT GAAT′ encoding the following 11-mer: glu gln lys leu ile ser glu glu asp leu asn. The polylinker sequence is: CTG CAG TCT AGA GTC GAC CTC GAG GGT CACC.

pSWI-VH-poly-tag1 (Ward, et al., 1989) was modified by the insertion of a unique NcoI site into the pelB leader sequence using site-directed dideoxynucleotide mutagenesis (Carter, et al., 1985) and the oligonucleotide 5′-GGC CAT GGC TGG TTG GG-3′ to generate VH Nco-poly-tag1. The pelB leader sequence was ATG AAA TAC CTA TTG CCT ACG GCA GCC GCT GGA TTG TTA TTA CTC GCT GCC CAA CCA GCG ATG GC. The underlined portion was converted to CCATGG by mutagenesis. The V_(α) gene isolated and tailored by the PCR was then cloned in translational frame as an NcoI-BstEII fragment into VH Nco-poly-tag1 to generate V_(α)pelBtag1 as shown in FIG. 3. Dideoxynucleotide sequencing was carried out to confirm the DNA sequences of the plasmid construction.

V_(β)pelBtag1 Plasmid

The V_(β)pelBtag1 plasmid was constructed according to the procedure for the V_(α) plasmid except that the V_(β) gene was used in place of V_(α). The construct is shown in FIG. 3.

V_(α)V_(β)pelBtag1 Plasmid

To construct the V_(α)V_(β)pelBtag1 plasmid, V_(α)pelBtag1 was modified by replacement of the 5′ HindIII site of pUC119 (Viera and Messing, 1987) with an EcoRI site by ligation of oligonucleotide V.5′-AGC TGA ATT C 3′ as a duplex into HindIII restricted V_(α)pelBtag1 (with the EcoRI site shown underlined). The ligation destroyed the HindIII site. It was then cloned as an EcoRI fragment into EcoRI restricted V_(β)pelBtag1, shown in FIG. 3. Recombinants were analyzed for correct orientation of the V_(α) gene with respect to the V_(β) gene by restriction enzyme analysis. Dideoxynucleotide sequencing was carried out to confirm the DNA sequences of the plasmid construction.

scV_(α)V_(β)pelBhis Plasmid

The V_(α) and V_(β) domains do not associate when they are co-expressed within the same bacterial cell. To drive the association of the two domains, therefore, the V_(α) domain was linked to the V_(β) domain by a synthetic peptide linker and the two domains expressed as a heterodimeric scTCR fragment. This heterodimer was expressed with carboxy-terminal (his)₆ peptide tags and purified as herein described for single chain domains. Purification yields were 0.5-1.0 mg/L culture.

The plasmid V_(α)V_(β)pelBmyc2 (FIG. 1) was constructed in a similar manner to V_(α)pelBmyc or V_(α)V_(β)pelBtag1, except that the V_(β)pelBmyc gene was cloned 3′ to the V_(α)pelBmyc gene. The plasmid ScV_(α)V_(β)PelBhis, shown schematically in FIG. 8, was constructed as indicated in FIG. 1. The V_(α) gene was ligated 5′ to the V_(β) gene so that in the expressed protein the V_(α) domain was located at the N-terminus of the V_(α)V_(β) heterodimer. Since the V_(α) domain is more soluble than the V_(β) domain and expressed at higher levels, this orientation of the two domains with respect to each other appears to assist in the secretion and folding of the scTCR.

The single chain linker, (Gly₄Ser)₃ (Huston et al., 1988) was ligated into BstEIII-PstI restricted V_(α)δV_(β)pelB as in the following DNA duplex:

5′-GTC AGC GGT GGA GGC GGT TCA GGC GGA GGT GGC TCT GGC GGT GGC 3′G CCA CCT CCG CCA ACT CCG CCT CCA CCG AGA CCG CCA CCG GGA TCG GAG GCT GCA-3′ CCT AGC CTC CG-5′

where the coding strand is indicated by underlining.

To construct sc V_(α)V_(β)pelBmyc, the resulting HindIII-PstI fragment (scV_(α)δV_(β)pelB) encoding the pelB leader, the V_(α) gene, the single chain linker and the 5′ end of the V_(β) gene was ligated into HindIII-PstI restricted V_(β)pelBmyc to replace the pelB leader. To insert the (his)₆ peptide tag into scV_(α)V_(β)pelBmyc, the plasmid was restricted with BstEII and the following duplex ligated into the construct:

5′-GTC ACC CAT CAC CAT CAC CAT CAC TAA TAA-3′ 3′G GTA GTG GTA GTG GTA GTG ATT ATT CAG TG-5′

with the coding strand indicated by underlining.

Recombinant clones with the correct orientation of the (his)₆ tag were identified by PCR screening. Ligation of the duplex in the correct orientation into BstEII cut scV_(α)V_(β)pelBmyc removed the 3′ BstEII site. In addition, the presence of 2 stop codons at the 3′ end of the histidine codons prevented readthrough into the downstream c-myc tag sequences. Nucleic acid and derived amino acid sequences of the single chain TCR with attached (his)₆ tag is shown in FIG. 7.

Single stranded DNA was purified from extruded phage using polyethylene glycol precipitation. Sequencing reactions were then carried out using aliquots of the single stranded DNA, appropriate oligonucleotide primers and Sequenase (USB Corp, Cleveland, Ohio 44122) as polymerase. Random low level incorporation of dideoxynucleotides corresponding to each nucleotide position in the gene which was being sequenced occurred by using low levels of chain terminators (dideoxynucleotides) in the reaction mixes. The extended, prematurely terminated single stranded DNA molecules were then analyzed by electrophoresis followed by autoradiography with radiolabeled nucleotides included in the reactions to improve the sensitivity of detection.

V_(α)pelBhis and V_(β)pelBhis were constructed using the strategy shown in FIG. 1. Prior to expression analysis, all DNA constructs were sequenced using the dideoxynucleotide method. Single stranded DNA was isolated from the clones by growth of the recombinant cells in the presence of helper phage, VCSM13 (Stratagene, La Jolla, Calif. 92037). Nucleic acid and derived amino acid sequences for V_(α)pelBhis and V_(β)pelBhis are shown in FIGS. 5 and 6 respectively.

V_(α)pelBhis Plasmid

V_(α)pelBhis was constructed using the strategy outlined in FIG. 1. This involved restriction by BstEII to remove the single chain linker sequence and the V_(β) domain gene followed by religation.

V_(β)pelBhis Plasmid

V_(β)pelBhis was constructed using the strategy outlined in FIG. 1. A PstI-EcoRI fragment encoding the majority of the V_(β) domain gene and the (his)₆ tag was isolated following restriction enzyme digestion. This was then ligated into PstI-EcoRI restricted V_(β)pelBmyc to replace the majority of the V_(β) gene and the c-myc tag.

Construction of scV_(α)V_(β)pelBHis ver. 2

A vector scV_(α)V_(β)pelBHis ver. 2 has been constructed in which two codons (val-thr) which are located between the 3′ end of the Jα gene and the 5′ end of the (gly4ser)₃ linker have been removed, FIG. 4. These codons are derived from the 3′ end of an immunoglobulin heavy chain variable domain, and may therefore interfere with the scTCR structure in the protein expressed from V_(α)V_(β)pelBHis. The two codons were removed using PCR mutagenesis, as shown in FIG. 4, and the following primers.

Primer A: 5′ GTA TCT GCA CCC TCC GAT CCG CCA CCG CCG CAT CCA CCT 3′ Primer B: 5′ ATC AGC ATC CAC CTC CGC CTG AAC CGC CTC CAC CCC GTT TAA TGG 3′

The scTCR encoded by scV_(α)V_(β)pelBHis ver. 2 can be secreted and purified in yields of 0.5-1 mg/liter of culture, and CD analysis indicates that the protein is folded into a similar, if not the same, structure as that encoded by sc V_(α)V_(β)pelBHis. Thus, for practical purposes of sc TCR production the two constructs do not appear to differ.

The nucleic acid and derived amino acid sequence of the single chain construct is shown in FIG. 9.

EXAMPLE 2

The following are examples of expression of V_(α), V_(β) and V_(α)V_(β) T-cell receptor domains employing E. coli hosts transformed with the vectors of Example 1.

Expression of V_(α), V_(β) and SCV_(α)V_(β) Proteins

Detection of unpurified recombinant protein in culture supernatants or osmotic shock fractions was performed as follows:

E. coli recombinants harboring V_(α)pelBmyc, V_(β)pelBmyc V_(α)V_(β)pelBmyc, or scV_(α)V_(β)PelBmyc were grown up in 2×TY (or 4×TY) plus 100 μl ampicillin and 1% (wt:vol) glucose to early stationary phase, pelleted by centrifugation, washed once in either 2×TY (or 4×TY) or 50 mM NaCl and then induced by resuspension in 2×TY (or 4×TY) plus 100 μg/ml ampicillin and 1 mM isopropyl-β-D-thiogalactopyranoside (IPTG) for 14-16 hrs. Cultures were grown and induced at 37° C. with shaking at 250 rpm. Culture supernatants were analyzed by western blotting (Towbin et al., 1979; Ward et al., 1985) using monoclonal antibody 9E10 (Evan et al., 1985) followed by anti-mouse F_(c) conjugated to horseradish peroxidase (ICN Immunobiologicals) for detection. Diamino benzidine (Sigma, St. Louis, Mo.) was used as the horseradish peroxidase substrate. To detect expressed proteins in osmotic shock fractions the following procedure was followed.

Recombinant cells harboring V_(α)pelBmyc, V_(β)pelBmyc, V_(α)V_(β)pelBmyc or scV_(α)V_(β)pelBmyc were grown up at 30° C. for 12-16 hours in the same media as above, pelleted by centrifugation and washed in either 4×TY or 50 mM NaCl, and resuspended in 4×TY plus 100 μg/ml ampicillin plus 0.1 mM IPTG plus 1 mg/ml leupeptin (a protease inhibitor) and 10 μg/ml PMSF for 5-6 hours. Periplasmic fractions were isolated using cold TES buffer or 20% TES as described below. Osmotic shock fractions (see below) were then analyzed using Western blotting and the 9E10 monoclonal antibody as above. FIG. 2 shows the results of western blotting for V_(α), V_(β) and scV_(α)V_(β) domains tagged with carboxy terminal c-myc.

For optimal yields of purified T-cell receptor proteins, recombinants harboring V_(α)pelBhis, V_(β)pelBhis and V_(α)V_(β)pelBhis were employed. 1-2 liter cultures of recombinants were grown up in 4×TY media (double strength 2×TY) plus 100 pg ampicillin/ml plus 1% (w/v) glucose for 15 hr at 30° C. Cells were pelleted by centrifugation, washed once in 4×TY and resuspended in 1 liter of 4×TY plus 100 μg ampicillin/ml, 0.1 mM-IPTG, 1 μg leupeptin/ml and 10 μg PMSF/ml and grown at 25° C. for 5-5.5 hrs. At this stage the majority of the recombinant protein was located in the periplasm, and was isolated by osmotically shocking the cells as follows:

Cells were cooled by standing on ice for 10 minutes, and then pelleted by centrifugation (6000 rpm, 15 minutes at 4° C.). Cell pellets were resuspended in cold (at 0-4° C.) 200 mM Tris-HCl pH 8.0, 500 mM sucrose and 0.5 mM Na₂EDTA (TES, 40 mls used per 1 liter culture). Cells were incubated in TES for 20-40 minutes at 0° C.), and then pelleted by centrifugation (10,000 rpm, 10 minutes at 4° C.). The supernatant was dialyzed against phosphate buffered saline overnight (3 changes at 4° C.). The pellets were resuspended in cold 20% v/v TES and incubated for 20-40 minutes at 0° C. The cells were again pelleted by centrifugation (10,000 rpm, 10 minutes at 4° C.), and the supernatant was dialyzed against phosphate buffered saline overnight (3 changes at 4°). Both TES and 20% TES supernatants were then passed through Ni²⁺-NTA-agarose columns. Bound protein was batch eluted in 1-2 ml fractions with 250 mM imidazole, pH 9.2. To reduce non-specific binding of additional proteins, the column was washed with 500 mM NaCl/100 mM Tris HCl, pH 8, and the same at pH 7.4, prior to elution. The purified protein was dialyzed extensively against 10 mM NaH₂PO₄, pH 7.0, prior to CD analysis. Purity of T-cell receptor fragments was assessed by 15% SDS/polyacrylamide gel electrophoresis followed by staining with Coomassie brilliant blue. The SDS-PAGE stained gel of the purified proteins is shown in FIG. 10.

Yields of the purified V_(α) and V_(β) domains were approximately 1-2 mg/L culture and 0.2 mg/L culture respectively. Crosslinking experiments with dithiobis(succimidylpropionate) (DSP) indicated that the V_(α) domain tended to form homodimers. To analyze the oligomeric state of the V_(α) domain, the purified protein (at a concentration of approximately 1 mg/ml in PBS) or culture supernatant from induced cultures (see above; 24 hours or more induction) was used. The purified protein was V_(α)(his)₆ (carboxy terminal (his)₆ tag) and the culture supernatant contained V_(α)myc (carboxy terminal c-myc tag). 50 μl of each sample was incubated with 0.1-2 mM of DSP for one hour at room temperature. The crosslinked samples were then analyzed by SDS-PAGE (under non-reducing conditions) followed by either staining with Coomassie brilliant blue (for V_(α)(his)₆) or Western blotting (for V_(α)myc). For the Western blotting, the V_(α) domain was detected using the 9E10 monoclonal antibody as above. For a significant proportion of V_(α) domain, the size following incubation with crosslinker was approximately 30 kDa, indicating the formation of homodimers.

As a single chain polypeptide, the 1934.4 hybridoma cell-derived scTCR with a (his)₆ peptide tag was secreted into the periplasm and purified using Ni²⁺-NTA-agarose in yields of about 0.5-1.0 mg/l culture. The lower growth and induction temperature and lower IPTG concentration (0.1 mM), about 25° C., was particularly beneficial in inducing higher expression yields for the single chain V_(α)V_(β) heterodimer.

An alternative purification of the single chain V_(α)V_(β) domain employed an antibody-linked sepharose column. The purification of the scTCR by affinity chromatography indicated epitopic recognition by the antibody employed, monoclonal antibody KJ16 which is specific for murine V_(βγ) (Kappler et al., 1988).

EXAMPLE 3

The following example illustrates that the expression vectors of Example 1 are not limited to expression in E. coli. Serratia marcescens was employed as host in the following example.

Expression and Secretion of TCR Single Chain TCRs from S. marcescens

The plasmid scV_(α)V_(β)pelBhis was transformed into S. marcescens by electroporation and transformants selected on 2×TY agar plates with 1 mg/ml ampicillin and 1% w/v glucose, or minimal media (Sambrook et al., 1989) plates plus 1 mg/ml ampicillin plus 1% w/v glucose. Transformants were grown up in minimal media plus 10% w/v casamino acids, 5% w/v glycerol, 0.5 mg/ml ampicillin (MCGA media) at 30° C. for 24 hrs with aeration (250 rpm). 30-50 ml of this culture was used to inoculate 500 ml of the same MCGA media and grown for 12-16 hrs overnight at 30° C. with aeration (250 rpm) and then IPTG added to a final concentration of 0.2-0.5 mM. Cells were induced for 12-24 hrs by growth at 30° C. with aeration (250 rpm) and then stood on ice for 10 min. Cells were pelleted by centrifugation for 30 min, 10,000 rpm, followed by 30 min at 14,000 rpm and the supernatant filtered through a 0.45 μm filter unit (Nalgene). The supernatant was concentrated 10-20 fold in a high pressure concentrator with a YM10 filter and then dialyzed overnight (3 changes) against PBS at 4° C. The dialyzed supernatant was passed through a Ni²⁺-NTA-agarose column using the procedure for isolation from E. coli host according to Example 2.

Alternatively, the S. marcescens recombinants may be induced for shorter time periods and the protein isolated from the periplasmic space by osmotic shocking. Yields are higher if longer induction periods are employed and the protein isolated from culture supernatant.

EXAMPLE 4

The folded state of the recombinant TCR fragments was assessed by circular dichroism (CD) analysis. Results indicated significant proportion of β-sheet structure, strongly suggesting native folding.

Circular Dichroism Analysis of Expressed TCR Proteins

FIG. 11 shows the circular dichroism spectra of recombinant TCR proteins. The recombinant TCR fragments were purified using the methodology described above, from E. coli cells harboring V_(α)pelBhis, V_(β)pelBhis and scV_(α)V_(β)pelBhis and dialyzed into 10 mM sodium phosphate pH7.0 prior to CD analysis. As a comparison, the immunoglobulin scFv fragment derived from the D1.3 antibody (Ward et al., 1989) was purified and used. This fragment was expressed from a plasmid construction derivative of pSW2 (Ward et al., 1989; McCafferty et al., 1990). The scFv was purified from the culture supernatant of induced cultures using lysozyme sepharose (Ward et al., 1989) and dialyzed against 10 mM sodium phosphate pH 7.0 prior to analysis in CD. The rationale for using this immunoglobulin fragment as a comparison is that molecular modeling indicates that the V_(α) and V_(β) domains of TCRs resemble immunoglobulin variable domains (Chethi et al., 1988; Novotny et al., 1986). For each recombinant TCR protein, several spectra were generated using different concentrations and/or protein from different purification batches. FIG. 5 shows representative spectra.

CD analyses were carried out using an AVIV model 60DS circular dichroism spectrophotometer at 25° C. and a cell path of 0.2 cm. Concentrations of proteins in 10 mM NaH₂PO₄ varied from 1.0 μM to 7.8 μM. Concentration of the purified proteins was determined by quantitative amino acid hydrolysis. Proteins examined were V_(α)(his)₆, V_(β)(his)₆, scTCRV_(α)V_(β)(his)₆ and D1.3scFv.

By comparison with both the CD spectrum of the structurally solved D1.3 Fv fragment (Bhat et al., 1990) and that of other proteins known to have a high proportion of β-pleated sheet structure, it was concluded that the CD spectra of the recombinant TCR fragments contained a high proportion of β-pleated sheet structure. This is consistent with the molecular modeling studies which indicate that the TCR domain fold is immunoglobulin-like in character (Chothia et al., 1988; Novotny et al., 1986). The features of the spectra are: i) a minima at 218 nm, indicative of β-pleated sheet structure, ii) no minima at the wavelengths which are characteristic of α helical structure (Johnson, 1990), implying that there is no a helical structure in the TCR domains which is consistent with molecular models, iii) no maxima at the wavelength expected for random coil structure (Johnson, 1990), indicating that at least the majority of the TCR domains are folded into secondary structure and not in denatured (unfolded) state, and iv) for the V_(α) domain, a maxima at 205 nm indicating the presence of β turns.

REFERENCES

The references listed below are incorporated herein by reference to the extent that they supplement, explain, provide a background for or teach methodology, techniques, and/or compositions employed herein.

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                   #             SEQUENCE LISTING <160> NUMBER OF SEQ ID NOS: 8 <210> SEQ ID NO 1 <211> LENGTH: 363 <212> TYPE: DNA <213> ORGANISM: Murine <400> SEQUENCE: 1 gactcagtga ctcagacgga aggtcaagtg gccctctcag aagaggactt tc #ttacgata     60 cactgcaact actcagcctc agggtaccca gctctgttct ggtatgtgca gt #atcccgga    120 gaagggccac agttcctctt tagagcctca agggacaaag agaaaggaag ca #gcagaggg    180 tttgaagcca catacaataa agaagccacc tccttccact tgcagaaagc ct #cagtgcaa    240 gagtcagact cggctgtgta ctactgcgct ctgagtgaaa actatggaaa tg #agaaaata    300 acttttgggg ctggaaccaa actcaccatt aaaccggtca cccatcacca tc #accatcac    360 taa                   #                   #                   #            363 <210> SEQ ID NO 2 <211> LENGTH: 120 <212> TYPE: PRT <213> ORGANISM: Murine <400> SEQUENCE: 2 Asp Ser Val Thr Gln Thr Glu Gly Gln Val Al #a Leu Ser Glu Glu Asp   1               5  #                 10  #                 15 Phe Leu Thr Ile His Cys Asn Tyr Ser Ala Se #r Gly Tyr Pro Ala Leu              20      #             25      #             30 Phe Trp Tyr Val Gln Tyr Pro Gly Glu Gly Pr #o Gln Phe Leu Phe Arg          35          #         40          #         45 Ala Ser Arg Asp Lys Glu Lys Gly Ser Ser Ar #g Gly Phe Glu Ala Thr      50              #     55              #     60 Tyr Asn Lys Glu Ala Thr Ser Phe His Leu Gl #n Lys Ala Ser Val Gln  65                  # 70                  # 75                  # 80 Glu Ser Asp Ser Ala Val Tyr Tyr Cys Ala Le #u Ser Glu Asn Tyr Gly                  85  #                 90  #                 95 Asn Glu Lys Ile Thr Phe Gly Ala Gly Thr Ly #s Leu Thr Ile Lys Pro             100       #           105       #           110 Val Thr His His His His His His         115           #       120 <210> SEQ ID NO 3 <211> LENGTH: 366 <212> TYPE: DNA <213> ORGANISM: Murine <400> SEQUENCE: 3 gaggctgcag tcacccaaag cccaagaaac aaggtggcag taacaggagg aa #aggtgaca     60 ttgagctgta atcagactaa taaccacaac aacatgtact ggtatcggca gg #acacgggg    120 catgggctga ggctgatcca ttattcatat ggtgctggca gcactgagaa ag #gagatatc    180 cctgatggat acaaggcctc cagaccaagc caagagaact tctccctcat tc #tggagttg    240 gctaccccct ctcagacatc agtgtacttc tgtgccagcg gtgatgcgtc gg #gagcagaa    300 acgctgtatt ttggctcagg aaccagactg actgttctgg tcacccatca cc #atcaccat    360 cactaa                  #                   #                   #          366 <210> SEQ ID NO 4 <211> LENGTH: 121 <212> TYPE: PRT <213> ORGANISM: Murine <400> SEQUENCE: 4 Glu Ala Ala Val Thr Gln Ser Pro Arg Asn Ly #s Val Ala Val Thr Gly   1               5  #                 10  #                 15 Gly Lys Val Thr Leu Ser Cys Asn Gln Thr As #n Asn His Asn Asn Met              20      #             25      #             30 Tyr Trp Tyr Arg Gln Asp Thr Gly His Gly Le #u Arg Leu Ile His Tyr          35          #         40          #         45 Ser Tyr Gly Ala Gly Ser Thr Glu Lys Gly As #p Ile Pro Asp Gly Tyr      50              #     55              #     60 Lys Ala Ser Arg Pro Ser Gln Glu Asn Phe Se #r Leu Ile Leu Glu Leu  65                  # 70                  # 75                  # 80 Ala Thr Pro Ser Gln Thr Ser Val Tyr Phe Cy #s Ala Ser Gly Asp Ala                  85  #                 90  #                 95 Ser Gly Ala Glu Thr Leu Tyr Phe Gly Ser Gl #y Thr Arg Leu Thr Val             100       #           105       #           110 Leu Val Thr His His His His His His         115           #       120 <210> SEQ ID NO 5 <211> LENGTH: 753 <212> TYPE: DNA <213> ORGANISM: Murine <400> SEQUENCE: 5 gactcagtga ctcagacgga aggtcaagtg gccctctcag aagaggactt tc #ttacgata     60 cactgcaact actcagcctc agggtaccca gctctgttct ggtatgtgca gt #atcccgga    120 gaagggccac agttcctctt tagagcctca agggacaaag agaaaggaag ca #gcagaggg    180 tttgaagcca catacaataa agaagccacc tccttccact tgcagaaagc ct #cagtgcaa    240 gagtcagact cggctgtgta ctactgcgct ctgagtgaaa actatggaaa tg #agaaaata    300 acttttgggg ctggaaccaa actcaccatt aaaccggtca ccggtggagg cg #gttcaggc    360 ggaggtggct ctggcggtgg cggatcggag gctgcagtca cccaaagccc aa #gaaacaag    420 gtggcagtaa caggaggaaa ggtgacattg agctgtaatc agactaataa cc #acaacaac    480 atgtactggt atcggcagga cacggggcat gggctgaggc tgatccatta tt #catatggt    540 gctggcagca ctgagaaagg agatatccct gatggataca aggcctccag ac #caagccaa    600 gagaacttct ccctcattct ggagttggct accccctctc agacatcagt gt #acttctgt    660 gccagcggtg atgcgtcggg agcagaaacg ctgtattttg gctcaggaac ca #gactgact    720 gttctggtca cccatcacca tcaccatcac taa        #                   #        753 <210> SEQ ID NO 6 <211> LENGTH: 250 <212> TYPE: PRT <213> ORGANISM: Murine <400> SEQUENCE: 6 Asp Ser Val Thr Gln Thr Glu Gly Gln Val Al #a Leu Ser Glu Glu Asp   1               5  #                 10  #                 15 Phe Leu Thr Ile His Cys Asn Tyr Ser Ala Se #r Gly Tyr Pro Ala Leu              20      #             25      #             30 Phe Trp Tyr Val Gln Tyr Pro Gly Glu Gly Pr #o Gln Phe Leu Phe Arg          35          #         40          #         45 Ala Ser Arg Asp Lys Glu Lys Gly Ser Ser Ar #g Gly Phe Glu Ala Thr      50              #     55              #     60 Tyr Asn Lys Glu Ala Thr Ser Phe His Leu Gl #n Lys Ala Ser Val Gln  65                  # 70                  # 75                  # 80 Glu Ser Asp Ser Ala Val Tyr Tyr Cys Ala Le #u Ser Glu Asn Tyr Gly                  85  #                 90  #                 95 Asn Glu Lys Ile Thr Phe Gly Ala Gly Thr Ly #s Leu Thr Ile Lys Pro             100       #           105       #           110 Val Thr Gly Gly Gly Gly Ser Gly Gly Gly Gl #y Ser Gly Gly Gly Gly         115           #       120           #       125 Ser Glu Ala Ala Val Thr Gln Ser Pro Arg As #n Lys Val Ala Val Thr     130               #   135               #   140 Gly Gly Lys Val Thr Leu Ser Cys Asn Gln Th #r Asn Asn His Asn Asn 145                 1 #50                 1 #55                 1 #60 Met Tyr Trp Tyr Arg Gln Asp Thr Gly His Gl #y Leu Arg Leu Ile His                 165   #               170   #               175 Tyr Ser Tyr Gly Ala Gly Ser Thr Glu Lys Gl #y Asp Ile Pro Asp Gly             180       #           185       #           190 Tyr Lys Ala Ser Arg Pro Ser Gln Glu Asn Ph #e Ser Leu Ile Leu Glu         195           #       200           #       205 Leu Ala Thr Pro Ser Gln Thr Ser Val Tyr Ph #e Cys Ala Ser Gly Asp     210               #   215               #   220 Ala Ser Gly Ala Glu Thr Leu Tyr Phe Gly Se #r Gly Thr Arg Leu Thr 225                 2 #30                 2 #35                 2 #40 Val Leu Val Thr His His His His His His                 245   #               250 <210> SEQ ID NO 7 <211> LENGTH: 747 <212> TYPE: DNA <213> ORGANISM: Murine <400> SEQUENCE: 7 gactcagtga ctcagacgga aggtcaagtg gccctctcag aagaggactt tc #ttacgata     60 cactgcaact actcagcctc agggtaccca gctctgttct ggtatgtgca gt #atcccgga    120 gaagggccac agttcctctt tagagcctca agggacaaag agaaaggaag ca #gcagaggg    180 tttgaagcca catacaataa agaagccacc tccttccact tgcagaaagc ct #cagtgcaa    240 gagtcagact cggctgtgta ctactgcgct ctgagtgaaa actatggaaa tg #agaaaata    300 acttttgggg ctggaaccaa actcaccatt aaaccgggtg gaggcggttc ag #gcggaggt    360 ggatccggcg gtggcggatc ggaggctgca gtcacccaaa gcccaagaaa ca #aggtggca    420 gtaacaggag gaaaggtgac attgagctgt aatcagacta ataaccacaa ca #acatgtac    480 tggtatcggc aggacacggg gcatgggctg aggctgatcc attattcata tg #gtgctggc    540 agcactgaga aaggagatat ccctgatgga tacaaggcct ccagaccaag cc #aagagaac    600 ttctccctca ttctggagtt ggctaccccc tctcagacat cagtgtactt ct #gtgccagc    660 ggtgatgcgt cgggagcaga aacgctgtat tttggctcag gaaccagact ga #ctgttctg    720 gtcacccatc accatcacca tcactaa           #                   #            747 <210> SEQ ID NO 8 <211> LENGTH: 248 <212> TYPE: PRT <213> ORGANISM: Murine <400> SEQUENCE: 8 Asp Ser Val Thr Gln Thr Glu Gly Gln Val Al #a Leu Ser Glu Glu Asp   1               5  #                 10  #                 15 Phe Leu Thr Ile His Cys Asn Tyr Ser Ala Se #r Gly Tyr Pro Ala Leu              20      #             25      #             30 Phe Trp Tyr Val Gln Tyr Pro Gly Glu Gly Pr #o Gln Phe Leu Phe Arg          35          #         40          #         45 Ala Ser Arg Asp Lys Glu Lys Gly Ser Ser Ar #g Gly Phe Glu Ala Thr      50              #     55              #     60 Tyr Asn Lys Glu Ala Thr Ser Phe His Leu Gl #y Lys Ala Ser Val Gly  65                  # 70                  # 75                  # 80 Glu Ser Asp Ser Ala Val Tyr Tyr Lys Ala Le #u Ser Glu Asn Tyr Gly                  85  #                 90  #                 95 Asn Glu Lys Ile Thr Phe Gly Ala Gly Thr Ly #s Leu Thr Ile Lys Pro             100       #           105       #           110 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gl #y Gly Gly Gly Ser Glu         115           #       120           #       125 Ala Ala Val Thr Gly Ser Pro Arg Asn Lys Va #l Ala Val Thr Gly Gly     130               #   135               #   140 Lys Val Thr Leu Ser Lys Asn Gly Thr Asn As #n His Asn Asn Met Tyr 145                 1 #50                 1 #55                 1 #60 Trp Tyr Arg Gly Asp Thr Gly His Gly Leu Ar #g Leu Ile His Tyr Ser                 165   #               170   #               175 Tyr Gly Ala Gly Ser Thr Glu Lys Gly Asp Il #e Pro Asp Gly Tyr Lys             180       #           185       #           190 Ala Ser Arg Pro Ser Gly Glu Asn Phe Ser Le #u Ile Leu Glu Leu Ala         195           #       200           #       205 Thr Pro Ser Gly Thr Ser Val Tyr Phe Lys Al #a Ser Gly Asp Ala Ser     210               #   215               #   220 Gly Ala Glu Thr Leu Tyr Phe Gly Ser Gly Th #r Arg Leu Thr Val Leu 225                 2 #30                 2 #35                 2 #40 Val Thr His His His His His His                 245 

What is claimed is:
 1. A cloning vector which expresses and secretes a soluble V_(α) or V_(β) T-cell receptor variable domain, said vector comprising the following elements in the 5′ to 3′ direction, said elements which are operatively linked: (a) a promoter DNA sequence; (b) a leader sequence; and (c) a DNA sequence encoding V_(α) or V_(β) T-cell receptor variable domain.
 2. The cloning vector of claim 1, further comprising an inducible promoter DNA sequence.
 3. The cloning vector of claim 1, further comprising a DNA sequence encoding a tag sequence, said tag sequence positioned 3′ to the DNA encoding said T-cell receptor variable domain.
 4. The cloning vector of claim 1, wherein the DNA encodes V_(α) T-cell receptor variable domain and V_(β) T-cell receptor variable domain.
 5. The cloning vector of claim 4, wherein the DNA sequence encoding the V_(α) T-cell receptor variable domain is 5′ to the DNA sequence encoding the V_(β) T-cell receptor variable domain.
 6. The cloning vector of claim 3, wherein the tag is myc or his.
 7. A eukaryotic cell transformed by the cloning vector of claim
 1. 8. A method for expressing and secreting a T-cell receptor variable domain in a host cell, comprising the steps: (a) culturing said host cell with a vector, said vector comprising the following elements in the 5′ to 3′ direction, said elements which are operatively linked: (i) a promoter DNA sequence; (ii) a leader sequence; and (iii) a DNA sequence encoding a V_(α) or V_(β) T-cell receptor variable domain; and (b) inducing said promoter; to produce a T-cell receptor variable domain.
 9. The method of claim 8, wherein the promoter DNA sequence is an inducible promoter DNA sequence.
 10. The method of claim 8, wherein the T-cell receptor variable domain is V_(α), V_(β), V_(γ) V_(δ) single chain V_(α)V_(β), scV_(β)V_(α, or scV) _(δ)V_(γ).
 11. The method of claim 8, expression of the T-cell receptor variable domain is induced in a culture medium.
 12. The method of claim 10, further comprising obtaining the expressed T-cell receptor variable domain.
 13. The method of claim 12, wherein T-cell receptor variable domain is obtained from the culture medium supernatant.
 14. The method of claim 12, wherein the expressed T-cell receptor variable domain is obtained by a process that includes an osmotic shock step.
 15. The method of claim 12, further comprising purifying the T-cell receptor domain by affinity metallic resin chromatography.
 16. The method of claim 15, wherein the metallic resin comprises Ni²⁺NTA.
 17. The method of claim 10, wherein the leader sequence comprises the pelB, ompA or phoA leader sequence.
 18. The method of claim 17, wherein the leader sequence comprises the pelB sequence.
 19. The method of claim 8, wherein said inducible promoter comprises the lacZ promoter and the inducer is isopropylthiogalactopyranoside.
 20. The method of claim 8, wherein expression of the T-cell variable domain is induced by the addition of about 0.1 to about 1 mM of isopropylthiogalactopyranoside.
 21. The method of claim 8, wherein the host cell is a eukaryotic cell.
 22. The method of claim 8, wherein said vector is further defined as comprising a tag sequence, said tag sequence positioned 3′ to the DNA encoding said T-cell receptor variable domain.
 23. A recombinant T-cell receptor single chain variable domain α, β heterodimer produced by the method of claim
 8. 